%0 Journal Article
%T Resolution of the Epinephrine Enantiomers Derivatized with Fluorescent Chiral Reagent by Reversed-Phase High Performance Liquid Chromatography<br>手性荧光衍生化反相高效液相法分离肾上腺素对映体
%A JIN Dong ri
%A MA Kui rong
%A ZHANG Xiao yong
%A WU Xin zi
%A CUI Sheng yun
%A ZHU Yu lan
%A TOYO''''OKA Toshimasa
%A <br>金东日
%A 马奎蓉
%A 张小勇
%A 吴信子
%A 崔胜云
%A 朱玉兰
%A 丰冈利正
%J 色谱
%D 2002
%I 
%X A new method was developed to separate epinephrine enantiomers derivatized with fluorescent chiral reagent, R-(-)/S-(+)-4-(N, N-dimethylaminosulfonyl)-7-(3-iso-thiocyanatopyrrolidino)-2, 1, 3-benzoxadiazole (DBD-PyNCS), by reversed-phase high performance liquid chromatography, on a Diamonsil C18 column(150 mm x 4.6 mm i.d., 5 microns), with a mobile phase of water-acetonitrile(72:28, volume ratio) and a fluorescent detector at the excitation wavelength of 460 nm and the emission wavelength of 550 nm. The column was eluted at 40 degrees C, with a flow rate of 1 mL/min. At first, let the fluorescent chiral reagent(36 mmol/L, 10 microL) react with epinephrine enantiomers(1 mmol/L, 10 microL), and then 10 microL 20% pyridine acetonitrile solution was added. The resulting mixture was stirred for 1 min and kept at 65 degrees C for 35 min and then a 10 microL aqueous of the diastereomeric derivatives was injected directly into the chromatograph. The diastereomeric compounds were efficiently separated. The Rs value was 2.6 with alpha of 1.07. The k's of L-epinephrine and D-epinephrine were 17.11 and 18.13 respectively. The retention times of L-epinephrine and D-epinephrine were 30.24 min and 31.94 min respectively. The linear range was 0.07 g/L -0.50 g/L (r = 0.9995). The RSD was 4.3%(n = 7). The detection limit was 12 g/L(S/N = 3).
%K fluorescent chiral derivatization reagent
%K reversed
%K phase high performance liquid chromatography
%K epinephrine enantiomer<br>分离
%K 手性荧光衍生化试剂
%K 反相高效液相色谱法
%K 肾上腺素对映体
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=501E4C31B14D6DDA&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=E158A972A605785F&sid=8ED630AD8C61FAE8&eid=F416A9924F23B020&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=8