%0 Journal Article
%T Two-Step Chromatographic Method for the Separation and Purification of Recombinant Angiogenesis Inhibitor Kringle 5<br>两步柱色谱法分离纯化重组肿瘤血管生长抑制因子Kringle 5
%A JIA Xingui
%A BIAN Liujiao
%A <br>贾信贵
%A 边六交
%J 色谱
%D 2007
%I 
%X The Kringle 5 domain of plasminogen,which was previously shown to inhibit angiogenesis in vitro and in vivo,is one of the most potent angiogenesis inhibitors known to date.A two-step chromatographic method,which consists of Chelating Sepharose Fast Flow chelated with Ni2+ affinity medium and Sephadex G-75 medium,was established to separate and purify recombinant angiogenesis inhibitor Kringle 5(rK5).Through the two-step chromatographic purification process,the obtained rK5 was confirmed to be homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and its relative molecular mass was estimated to be about 32 000,which matched well with the prediction by gene sequence.Its purity was about 98%,and the total protein recovery of this method was 0.63%.In addition,it was found that it inhibited the blood vessel growth of chick embryo chorioallantoic membrane effectively.
%K affinity column chromatography
%K gel exclusion column chromatography
%K recombinant angiogenesis inhibitor Kringle 5
%K separation
%K purification<br>亲和柱色谱法
%K 凝胶排阻柱色谱法
%K 重组血管生长抑制因子
%K Kringle
%K 5
%K 分离
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F27543609C24D938&yid=A732AF04DDA03BB3&vid=C5154311167311FE&iid=38B194292C032A66&sid=A04F01817ECB9A48&eid=15251AE9C02726D3&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=12