%0 Journal Article
%T Determination of Glutathione in a Single Human Hepatocarcinoma Cell Using a Microfluidic Device Coupled with Electrochemical Detection<br>微流控芯片电泳/电化学检测人单个肝癌细胞中的谷胱甘肽
%A WANG Wenlei
%A JIN Wenrui
%A <br>王文雷
%A 金文睿
%J 色谱
%D 2007
%I 
%X A method for the determination of glutathione(GSH) in a single human hepatocarcinoma cell,using a microfluidic chip coupled with electrochemical detection,was developed.In this method,the cell injection,loading,and cytolysis,as well as the transportation and detection of intracellular GSH were integrated in a microfluidic chip with a doubme-T injector and an end-channel amperometric detector.A single cell was loaded in the double-T injector on the microfluidic chip,using liquid pressure.The docked cell was perforated in the electrophoretic buffer containing digitonin and was lysed under a direct current electric field.The GSH from the single cell was electrokinetically transported to the separation channel and was electrochemically detected at an Au/Hg electrode.The GSH in the single cell was quantitatively determined using a calibration curve.
%K microfluidic chip
%K electrochemical detection
%K glutathione
%K single cell
%K hepatocarcinoma cell<br>微流控芯片
%K 电化学检测
%K 谷胱甘肽
%K 单细胞
%K 肝癌细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=63F8F4FBFD00689421A6AD94D7219D21&yid=A732AF04DDA03BB3&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=5EEA08EFB4616D1C&eid=EAF6504CC0383BDF&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=27