%0 Journal Article %T Rapid Analysis of Terpene Lactones in Extract of Ginkgo biloba L.by High Performance Liquid Chromatography
高效液相色谱法快速测定银杏叶提取物中的萜类内酯 %A WANG Hai-feng %A JU Xing-rong %A
汪海峰 %A 鞠兴荣 %J 色谱 %D 2000 %I %X A new rapid analytical method was developed for the quantification of terpene lactones (bilobalide(BB) and ginkgolide A, B, C, J) in extract of Ginkgo biloba L. (EGb) using a liquid-liquid solvent extraction procedure followed by high performance liquid chromatography. EGb was dissolved in 30% ethanol and extracted with ether. After evaporation, the residue was then determined by HPLC on a C18 column with methanol-water-orthophosphoric acid (25:75:0.1, V/V) as eluent and refractive index (RI) detection. Results showed that the excellent sample clean-up procedure is more simple and specific, and saves more time (less than 20 min) than any other methods that have been reported, and also leads to high recoveries (> 99.0%) and low RSDs (< 2.0%). The reproducible method is regarded to be very useful for evaluating the quality of extract of Ginkgo biloba L. %K high performance liquid chromatography %K rapid analysis %K terpene lactone %K extract of %K Ginkgo biloba %K L
高效液相色谱法 %K 萜类内酯 %K 银杏叶提取物 %K 分析 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=0319B9BD3BDAAF56&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=94C357A881DFC066&sid=0BD4FAD4A90498AB&eid=21D8CE17EE5EE354&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=0