%0 Journal Article
%T Simultaneous Analysis of Histamine and Noradrenaline in Human Plasma by High Performance Liquid Chromatography/Electrochemical Detection<br>高效液相色谱-电化学检测法同时测定人血浆中的组织胺和去甲肾上腺素
%A Liu Wendi
%A Qi Wei
%A <br>刘文弟
%A 齐伟
%A 郑惠良
%J 色谱
%D 1999
%I 
%X This paper reports a reversed-phase high performance liquid chromatographic method for the simultaneous separation and determination of histamine(Hist) and noradrenaline(NA) in human plasma by means of pre-column derivatization of Hist and NA with OPA in the presence of 2-mercaptoethanol. The analysis was carried out with Partisil ODS column, Vsodium acetate buffer solution(pH 4.90)]:V(acetonitrile) = 7:3 as mobile phase and 3-methylhistamine as internal standard. The detector potential was +0.7 V for both compounds to keep minimum background noise. A linear relationship between the electrochemical signal and the compound mass concentrations was obtained. The minimum detectable limit was 15 ng/L (S/N = 3). An example of application of the method to the determination of Hist and NA in human plasma is presented.
%K high performance liquid chromatography
%K electrochemical detection
%K histamine
%K noradrenaline
%K pre column derivatization<br>高效液相色谱法，电化学检测器，组织胺，去甲肾上腺素，柱前衍生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862DBEC0766811F343F&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=CA4FD0336C81A37A&sid=E203FB1A272C9DD2&eid=0D0D661F0B316AD5&journal_id=1000-8713&journal_name=色谱&referenced_num=14&reference_num=1