%0 Journal Article
%T The Determination of Vitamin D in Fortified Milk and Other Foods by High Performance Liquid Chromatography(HPLC)<br>高效液相色谱法测定强化奶及食品中维生素D含量
%A Wu Huaichun
%A Cheng Hua
%A Tian Jiarong
%A Wang Xisheng
%A <br>吴怀春
%A 程华
%A 田嘉荣
%A 王喜生
%J 色谱
%D 1997
%I 
%X An HPLC procedure for determination of vitamin D(VD) in fortified milk and food has been developed. The samples were saponified by adding KOH, ethanol and vitamin C at 75 degrees C for 30 min. The digest was extracted with hexane in multistep manner. The extract was washed with 5% KOH, distilled water and 55% ethanol, and evaporated under reduced pressure at 50 degrees C. The residue was dissolved in hexane, transferred to a coentrifuge tube, evaporated under N2 and then dissolved in 200 microL mobile phase. The latter was injected into a silica column (10 microm, 30 cm x 4 mm) for cleanup, eluted with mixture of cyclohexane-hexane containing 0.8% isopropanol and detected at UV 254nm. VD fraction was collected according to the retention time of standard, evaporized under N2, redissolved in 100 microL MeOH, and separated on a C18 column (10 microm, 30 cm x 4 mm) with MeOH/H2O (98:2) as eluent. VD2 and VD3 were eluted as a mixture under the conditions. Recoveries of the method were 94.8% to 99.7%. The average CV (n = 5) for within and between day measurements were 1.6% and 2.1% respectively. This method was already used to determine the content of vitamin D in milk powder, pulvis elementi compound, mixed proteins, dried meat floss, humanized milk power, children health drink, milk wheat powder and fortified milk. The method is simple, rapid and accurate.
%K high
%K performance
%K liquid
%K chromatography
%K vitamin
%K D
%K fortified
%K milk
%K food<br>高效液相色谱法
%K 维生素D
%K 强化奶
%K 食品
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D772C9CD788EEF6FA&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=CA4FD0336C81A37A&sid=BE33CC7147FEFCA4&eid=94E7F66E6C42FA23&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=2