%0 Journal Article
%T Purification of Pig Copper,Zinc-Superoxide Dismutase by Immobilized Metal Ion Affinity Chromatography<br>固定化金属离子亲和色谱法纯化猪铜锌超氧化物歧化酶
%A Shao Tianmeng
%A :Liu Yuxin
%A Shao Changping
%A <br>邵天梦
%A 刘宇新
%A 邵昌平
%J 色谱
%D 1996
%I 
%X Copper,zinc superoxide dismutase (Cu,Zn-SOD,EC,1.15.1.1)catalyzes the dismutation of the superoxide radical produce O2,and avoid destroying organisms,Cu,Z-SOD has been used experimentally and clinically as a therapeutic drug.It is usually purified by precipitation with ammonium sulphate, ion -exchange chromatography and gel filtration. However the yields and extent of purification are relatively low.The development of immobilized metal ion affinity chromatography offers a new approach to enzyme purification.Iminodiacetic acid (IDA)was coupled to epoxy-activated Sepharose 4B.The column of IDA-epoxy-Sepharose 4Bwas activated by copper sulfate solution.Cu,Zn-SOD solution was applied to the column which had been equilibrated with phosphate buffer, then eluted with (1)buffers of phosphate and acetate containing imidazole and (2)buffers of phosphate and Tris-HCl, respectively.The results show that (1) high purification fold (38.4) with activity recovery over 90% for crude Cu,Zn-SOD solution using Cu2 -Sepharose 4B column was obtained,much more than those of DEAE-52 ionexhange column,(2)elution efficiency of the buffers of phosphate and Tris -HCl is better for the enzyme solution having more proteinic impurities, while the buffers of phosphate and acetate containing imidazole are fit for the enzyme solution having fewer proteinic impurities.
%K immobilized metal ion affinity chromatography
%K pig-superoxide dismutase<br>离子亲和色谱
%K 猪
%K 超氧化物歧化酶
%K 色谱
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=473278D7608BFF3E965499CFB242D56A&yid=8A15F8B0AA0E5323&vid=F3583C8E78166B9E&iid=38B194292C032A66&sid=AD16A18DBD734D13&eid=78F0EFE028BD3783&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=1