%0 Journal Article
%T Preparation and Evaluation of Polyvinyl Pyrrolidone-Coated Columns Based on Sol-Gel Chemistry for Capillary Electrophoresis<br>基于溶胶凝胶技术的聚乙烯吡咯烷酮涂层柱的制备及评价
%A ZHANG Jin-lan
%A ZHOU Tong-hui
%A <br>张金兰
%A 周同惠
%J 色谱
%D 2000
%I 
%X Polyvinyl pyrrolidone(PVP) coating techniques based on sol-gel chemistry was investigated in this article. Multiple-step coating and single-step coating methods were developed and the coating conditions(molar ratios of MAPS to 0.1 mol/L HCl, reaction times and concentration of PVP) were selected. Electroosmosis mobility and column efficiency were used as the evaluating parameters for coated capillary columns. For multiple-step coating, the optimal sublayer sol solution was obtained by adding 0.1 mol/L hydrochloric acid to MAPS(molar ratio, 1:1) and reacted at room temperature for 2 hours. The best PVP mass concentration was 40 g/L in alcohol. For single-step coating, the sol-gel solution was obtained by mixing the following ingredients: (a)MAPS, (b) 8% PVP alcohol solution and (c) 0.1 mol/L hydrochloric acid in 1:1 molar ratio to MAPS. The mixture was kept at room temperature for 2 hours. Proper amounts of 100 g/L ammonium persulfate and 10% (V/V) N, N, N', N'-tetramethylethylenediamine were then added. The mixture was mixed ultrasonically, then centrifuged at 4,000 r/min for 5 min. The upper clean sol-gel solution was transferred into another clean vial for coating capillary column. The reproducibility of migration times and efficiencies of single-step-coated capillary was better than that of multiple-step-coated capillary. Single-step-coating method was very effective and easy. It can fit the needs in experimental work. Coating techniques based on sol-gel chemistry are prospective but still need to be further developed.
%K NULL<br>涂层柱
%K 聚乙烯吡咯烷酮
%K 溶胶－凝胶
%K 毛细管电泳
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=9BA2AC08419A7FD8696917FBE29D451F&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=DDDA4F26E8AD3C0E&eid=A03A15CF5604A8B0&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=3