%0 Journal Article
%T Determination of bromhexine in plasma by gas chromatography-electron capture detection and pharmacokinetic studies]<br>气相色谱法测定溴己新血药浓度及药代动力学研究
%A L L Yang
%A Y S Yuan
%A X D Tu
%A <br>杨丽莉
%A 袁倚盛
%A 屠锡德
%J 色谱
%D 2000
%I 
%X This paper reports a gas chromatography-electron capture detection(GC-ECD) method for the study of pharmacokinetics of bromhexine in healthy human body. A2 m x 3 mm i.d. silanized glass column packed with 5% SE-30 was used. The carrier gas was nitrogen. The internal standard was 5-chloro-2-amino-diphenyl ketone. After NaH2PO4-Na2HPO4 buffer (pH 6.0) being added, the plasma was extracted with n-hexane-dichloromethane (9:1, V/V). A good linearity was obtained from 1.0 microgram/L to 50.0 micrograms/L of bromhexine in human plasma, r = 0.9994. The detection limit of bromhexine in plasma was 0.5 microgram/L. The average recovery was 97.5%. The pharmacokinetics of bromhexine was determined by this method after a single oral dose of 8 mg bromhexine capsule given to each of 8 volunteers. The results showed that the plasma concentration-time courses conformed to one compartment model. The established GC-ECD method is a good method for the determination of bromhexine in human plasma. The method is rapid, simple, precise and sensitive.
%K NULL<br>溴己新
%K 药代动力学
%K 血药浓度
%K 测定
%K GC－ECD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=1915AE01FC486B77936CAD65DFED422A&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=BA305A52E2EE9350&eid=6313C162FF75889A&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=2