%0 Journal Article
%T Determination of Ligustrazine in Human Plasma by High Performance Liquid Chromatography with<br>固相萃取-高效液相色谱法测定人血浆中的川芎嗪
%A WENG Weiyu
%A SHEN Teng
%A XU Huinan
%A ZHANG Jianfang
%A HUANG Jianming
%A HUANG Zhaochang
%A CAI Jia
%A <br>翁伟宇
%A 沈腾
%A 徐惠南
%A 张建芳
%A 黄建明
%A 黄照昌
%A 蔡佳
%J 色谱
%D 2005
%I 
%X A high performance liquid chromatographic method was developed for the determination of ligustrazine in human plasma. The chromatographic separation was performed on a Luna C18 column (150 mm x 4.6 mm i.d., 5 microm) at column temperature of 40 degrees C. The mobile phase, a mixture of methanol-acetonitrile-acetate buffer of pH 5.0 (50:8:42, v/v), was delivered at a flow rate of 1.0 mL/min. The detection wavelength was 280 nm. Plasma samples were prepared with a C8 solid-phase extraction column. Linearity was confirmed in the mass concentration range of 25-5000 microg/L with the correlation coefficient of 0.9999. The extraction recovery of ligustrazine ranged from 96.72% to 100.90%. The relative standard deviations (RSDs) of intra- and inter-day assay at the mass concentrations of 50, 500 and 3000 microg/L were less than 8.64% and the accuracies were between 99.59%-103.26%. The limit of detection (LOD) was 10 microg/L. The results of this method validation satisfactorily meet the acceptance criteria of bioanalysis and the method is applicable to the pharmacokinetic studies of ligustrazine in human beings.
%K high performance liquid chromatography
%K ligustrazine
%K human plasma<br>反相高效液相色谱法
%K 川芎嗪
%K 人血浆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=14739655A0C4085A&yid=2DD7160C83D0ACED&vid=EA389574707BDED3&iid=38B194292C032A66&sid=44A4891E33BFF455&eid=B4E8EA49DAAEB84F&journal_id=1000-8713&journal_name=色谱&referenced_num=7&reference_num=6