%0 Journal Article
%T Determination of metronidazole in serum and cerebrospinal fluid with reversed-phase high performance liquid chromatography]<br>RP-HPLC测定兔血清和脑脊液中甲硝唑含量
%A X Zhang
%A H Zhou
%A W Li
%A J Chen
%A <br>张?
%A 周宏兵
%A 李伟
%A 陈坚
%J 色谱
%D 1997
%I 
%X A reversed-phase high performance liquid chromatographic method for the determination of metronidazole (MTZ) in rabbit serum and cerebro-spinal fluid has been developed. The method is simple and convenient. The analysis was carried out with Zorbax C8 column, NaH2PO4(0.0075mol/L, pH 4.0)-CH3OH(4:11) as mobile phase and UV detector at 320nm, by using hydrochlorothiazide as internal standard. The standard curve is linear over the range from 0.5ng/L to 80.0ng/L in sample with correlation coefficient of 0.9998. The recovery of metronidazole from rabbits serum and cerebrospinal fluid are 97.1% +/- 1.5% and 97.4% +/- 1.6% respectively. The minimum detectable quantity is 80ng/mL in sample (S/N = 3). Under the chromotographic condition, the consistency of MTZ of the serum and cerebrospinal fluids of rabbits were detected separately after taking MTZ (0.1mg/g) orally. The drug-time curve was obtained and a satisfactory result was achieved. The new findings provide conditions for further exploration of the process of pharmacokinetics.
%K 高效液相色谱法
%K 甲硝唑
%K 血清
%K 脑脊液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D496EC344450CADB5&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=0B39A22176CE99FB&sid=C5F8B8CB20F1B3D8&eid=9D453329DCCABB94&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=4