%0 Journal Article
%T Determination of Triptolide in Extract from Leigongteng (Tripterygium Wilfordii Hook. F.) by RP HPLC<br>反相高效液相色谱法测定雷公藤提取物中雷公藤甲素含量
%A K Li
%A Y Yuan
%A X Dai
%A X Qao
%A <br>李克
%A 袁倚盛
%A 戴晓莉
%A 乔小云
%J 色谱
%D 1998
%I 
%X A reversed-phase liquid chromatographic method has been developed and validated for the analysis of triptolide in extract from Leigongteng (Tripterygium Wilfordii Hook. F.). The drugs were eluted with petroleum-ethyl acetate on a neutral alumina cartridge and then measured by HPLC using a Hypersil C18 column and acetonitrile-water (40:60, V/V) mobile phase. Triptolide was quantified by ultraviolet absorbance at 210 nm. The method proved to be linear in the range of 0.25-4.00 mg/L with a regression coefficient of 0.9997. The lower limit of detection of triptolide was 0.1 mg/L. Intra-day and inter-day coefficients of variation of assay for triptolide were 1.9%-6.5% (n = 5) and 2.7%-7.8% (n = 5), respectively. The recoveries of the method were 89.8%-92.0%.
%K igh performance liquid chromatography
%K triptolide
%K pharmaceutical analysis<br>雷公藤甲素
%K 分析
%K 提取物
%K HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D9D03FAE834C0D564&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=E158A972A605785F&sid=35E8A259891FB32F&eid=FA88DCCE84EA0A56&journal_id=1000-8713&journal_name=色谱&referenced_num=12&reference_num=8