%0 Journal Article
%T Separation and Determination of Ephedrine Alkaloids and 2,3,5,6- Tetramethyl Pyrazine in Ephedra Herba by HPLC<br>高效液相色谱法测定麻黄及其制剂中的麻黄类生物碱和川芎嗪
%A LI Hong-xia
%A DING Ming-yu
%A L Kun
%A YU Jian-yuan
%A <br>李红霞
%A 丁明玉
%A 吕琨
%A 郁鉴源
%J 色谱
%D 2001
%I 
%X A sensitive and reliable high performance liquid chromatographic method(HPLC) has been developed for the first time for the simultaneous determination of the active ingredients of ephedrine alkaloids and 2,3,5,6-tetramethyl pyrazine (TMP) in Ephedra herba crude drug and two Chinese traditional medicines (Xiao-er qingfeiwan and Lu-si kewan). The HPLC assay was performed on a reversed phase C18 column (Nova-Pak C18, 3.9 mm i.d. x 150 mm) by using methanol-0.02 mol/L KH2PO4-acetic acid-triethyl amine (4:96:0.2:0.01, V/V) as mobile phase for the ephedrine alkaloids analysis and methanol-H2O-acetic acid (35:65:0.5, V/V) as mobile phase for TMP analysis. Regression equations revealed the linear relationships (correlation coefficients: 0.991-0.998) between the peak area of each constituent (E, PE, NE, NPE, TMP) and its concentration. The detection limits for E, PE, NE, NPE and TMP were 0.4 mg/L, 0.1 mg/L, 0.03 mg/L, 0.02 mg/L and 0.03 mg/L, respectively, and the recoveries ranged between 92%-103%. The contents of E, PE, NE, NPE, TMP in Ephedra herba, traditional medicine Xiao-er qingfeiwan and Lu-si kewan were determined respectively. The relative standard deviations (RSD) of the contents ranged between 1.1%-3%.
%K high performance liquid chromatography
%K ephedrine(E)
%K norephedrine(NE)
%K pseudoephedrine(PE)
%K norpseudoephedrine(NPE)
%K 2
%K 3
%K 5
%K 6
%K tetramethyl pyrazine (TMP)
%K Ephedra<br>高效液相色谱法
%K 麻黄碱
%K 伪麻黄碱
%K 去甲基麻黄碱
%K 去甲基伪麻黄碱
%K 川芎嗪
%K 麻黄
%K 中药分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=BA6291FC93A60543&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=0B39A22176CE99FB&sid=8575BEDA702C4B7C&eid=D5C9DC4EF2F78008&journal_id=1000-8713&journal_name=色谱&referenced_num=24&reference_num=7