%0 Journal Article
%T Study on the Retention Behaviors of Eight Pyrazines by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法对八种吡嗪类化合物保留性能的研究
%A You Jinmao
%A Sun Xuejun
%A Zhu Qingcun
%A <br>尤进茂
%A 孙学军
%A 朱庆存
%A 郑庚修
%J 色谱
%D 1996
%I 
%X The separating condition of eight pyrazines by reversed-phase high performance liquid chromatography with methanol/water system as eluant has been studied on Spherisorb-C(18) column. The results showed that the capacity factor(k')of eight compounds decreases with the increase of concentration of methanol. When it is higher than 40% (V/V), the separation of the early peaks can not be achieved. When it is less than 40% (V/V),the retention time of the later eluted components is up to 30-45 minutes. Inorganic salts or micro amount of organic amine can improve the separation. In particular, the analytical time can be shortened obviously by adding triethylamine modifier in mobile phase. A mixture of methanol:0. 2mol/L HAc-NaAc buffer solution (pH 4. 5):triethylamine=40:59:1(V/V) was used as mobile phase with UV 254 nm detection. All components ,except 2, 3- and 2 , 5-dimethylpyrazines , were separated within 25 minutes.
%K reversed-phase high performance liquid chromatography
%K pyrazines
%K retention performance<br>反相高效液相色谱法，吡嗪类化合物，保留性能
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=6E93C5880F5B87E4B4B72726E5E249AB&yid=8A15F8B0AA0E5323&vid=F3583C8E78166B9E&iid=E158A972A605785F&sid=B8F8200D88DDC7D6&eid=EBD6B792C9111B87&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=0