%0 Journal Article
%T Determination of L-Cysteine in Enzymatic Reaction Mixture with Pre-column Derivatizatization by High Performance Liquid Chromatography<br>5,5′-二硫硝基苯甲酸柱前衍生高效液相色谱法测定酶促反应液中的L-半胱氨酸
%A LIU Zhong
%A YANG Wenbo
%A JIN Yongjie
%A BAI Gang
%A <br>刘忠
%A 杨文博
%A 金永杰
%A 白钢
%J 色谱
%D 2004
%I 
%X A new sensitive high performance liquid chromatographic method for the determination of L-cysteine in an enzymatic reaction mixture using ultra violet spectrometric detection was developed. The sample reacted with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) and was analyzed on a Shimadzu VP-ODS column at room temperature, using gradient elution with detection at 330 nm. The L-cysteine chromatographic peak was determined in comparison with derivatives of 2-mercapto ethanol and dithiothreitol. The linear range was 5-950 micromol/L. The recoveries were 99.7%-100.5% and the relative standard deviations (RSDs) were less than 1.3%. The detection limit was 0.8 micromol/L. The method is simple and accurate.
%K NULL<br>高效液相色谱法
%K 5，5′-二硫硝基苯甲酸
%K 柱前衍生
%K L-半胱氨酸
%K 酶促反应液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=23D1EA49D7B14397&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=38B194292C032A66&sid=FA89360EB995A8AD&eid=FD7C952458BFB5D8&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=12