%0 Journal Article
%T Determination of Coenzyme Q and<br>液相色谱-电化学检测法测定小鼠血浆和组织线粒体中的辅酶Q和维生素E
%A QU Feng
%A LIN Jin ming
%A <br>屈锋
%A 林金明
%J 色谱
%D 2001
%I 
%X A convenient and reliable liquid chromatographic method with electrochemical detection was developed for the simultaneous determination of reduced coenzyme Q(ubiquinol), oxidized coenzyme Q(ubiquinone) and alpha-tocopherol in various tissues. The compounds of interest were separated on a reverse-phase C18 column under the mobile phase of 7 g/L NaClO4 in ethanol-methanol-70% HClO4 (800:200:1, volume ratio) and detected simultaneously by using a series of detection cells. Electrochemical guard cell (upstream of the injector) was used for eliminating interference for mobile phase. Ubiquinones were reduced in the -550 mV conditioning cell (downstream of the column). The resultant ubiquinols and original ubiquinols and alpha-tocopherol existing in the sample were easily oxidized in the 150 mV analytical cell. The linear range of the compounds were from 5 micrograms/L to 200 micrograms/L with correlation coefficients from 0.9976 to 0.9999. The detection limits were from 0.23 microgram/L to 0.68 microgram/L. The method was applied satisfactorily to the simultaneous determination of the amounts of ubiquinol-9, ubiquinol-10, ubiquinone-9, ubiquinone-10 and alpha-tocopherol in mice plasma and tissue mitochondria.
%K 液相色谱
%K 电化学检测
%K 辅酶Q
%K 维生素E
%K 血浆
%K 线粒体
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=E6BB237F2424F81B&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=B31275AF3241DB2D&sid=F176272DD933A0BB&eid=4A2356A1257A12EB&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=21