%0 Journal Article
%T Detection of 8-Hydroxyguanine in the DNA of the H_2O_2-Treated HL-60 Cells by GC/FID<br>气相色谱/氢火焰检测器检测HL-60细胞DNA中氧化损伤产物8-羟基鸟嘌呤的研究
%A Zhang Haitao
%A Zhu Qifeng
%A Mo Li'er
%A Zhuang Haiqi
%A Cat Chun
%A <br>张海涛
%A 祝其锋
%A 莫丽儿
%A 庄海旗
%A 蔡春
%J 色谱
%D 1999
%I 
%X This paper describes the DNA damage by hydrogen peroxide in HL-60 cells and the detection of 8-hydroxyguanine produced. The number of cells was at least about 2 x 10(7)/sample. Treatment of HL-60 cells with 0.4 mmol/L hydrogen peroxide for 24 h would result in DNA damage. The DNA samples were isolated, then qualitatively and quantitatively analyzed before hydrolysis. The results of experiments showed that 100-300 microL 98% formic acid could hydrolyze about 0.12 mg DNA samples completely in 150 degrees C for 1 h. After lyophilizing the DNA samples for 6-8 h, one DNA sample was trimethylsilylated completely with 150 microL BSTFA in 150 degrees C for 1 h. The recovery of trimethylsilylated 8-hydroxyguanine was 81.70%. The trimethylsilylated 8-hydroxyguanine was identified by GC/FID and CGC/MS-SIM parallely.
%K gas chromatography-flame ionization detector
%K gas chromatography-mass spectrometry
%K HL-60cell
%K 8-hydroxyguanine<br>气相色谱-氢火焰检测器
%K 气相色谱-质谱法
%K HL－60细胞
%K 8-羟基鸟嘌呤
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=BDD5337E0FF90E487BB9EE852599BCFF&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=38B194292C032A66&sid=30897FA31CA3354D&eid=7ABC4505E3960D2B&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=0