%0 Journal Article
%T Determination of Two Components in Chloramphenicol and Hydrocortisone Ear Drops by High Performance Liquid Chromatography<br>HPLC法测定氯霉素氢化可的松滴耳液中两组分的含量
%A Li Xiaoyan
%A <br>李小燕
%J 色谱
%D 1998
%I 
%X Chloramphenicol and hydrocortisone ear drops are possessed of anti-inflammatory and antiallergic functions. It can be used effectively in the treatment of tympanitis. It is composed of chloramphenicol and hydrocortisone. In this paper, we report a reversed phase HPLC method for the determination of chloramphenicol and hydrocortisone in the drops. The operatings conditions were YWG-C18 column (5 microns, 5 mm x 150 mm) at 30 degrees C, mobile phase of methanol-water (60:40, V/V) and UV detector at 244 nm. Prednisone acetate was selected as the internal standard. The linear relationship of calibration curves was good in the range of 40-200 and 8-40 mg/L for chloramphenicol and hydrocortisone, respectively (r = 0.9999). The average recovery and RSD of chloramphenicol and hydrocortisone were 99.91%, 0.86% and 99.58%, 1.34% (n = 6) respectively. The total time for separation and determination was within 9 min. The method was simple, sensitive, rapid and accurate.
%K igh performance liquid chromatography
%K chloramphenicol
%K hydrocortisone
%K chloramphenicol and hydrocortisone ear drops<br>氯霉素
%K 氢化可的松
%K 滴耳液
%K HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F65B1163FA62F63D41109DE5EC9CCB2A&yid=8CAA3A429E3EA654&vid=7801E6FC5AE9020C&iid=CA4FD0336C81A37A&sid=4F2F18DD6F870C2C&eid=B9704B40A4225A24&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=4