%0 Journal Article
%T The Analysis of Rice Total RNA by Capillary Electrophoresis
毛细管电泳技术分析水稻中的总核糖核酸
%A FAN Xing jun
%A JIANG Ge
%A JIN You xin
%A WANG De bao
%A
樊兴君
%A 江舸
%A 金由辛
%A 王德宝
%J 色谱
%D 2001
%I
%X When the RNase was eliminated through backing at 200 degrees C or treated by diethylpyrocarbonate (DEPC), the total RNA of rice could be separated within 15 min using 1.0%T, 0%C linear polyacrylamide as sieving matrix and 7 mol/L urea as denaturant. The tRNA of rice can be separated into two classes and about nine peaks when high concentrated polyacrylamide sieving matrix (5.0%T, 0%C) was used. This technique could provide one of the rapid and accurate methods for the determination of RNA in plants.
%K capillary electrophoresis
%K rice total RNA
%K rice tRNA
毛细管电泳
%K 水稻
%K 总RNA
%K 转移RNA
%K 分析
%K 生物化学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8FA4B8C44E8D375C&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=0B39A22176CE99FB&sid=ED01F5AE50BE09C0&eid=954CE65414DD94CA&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=6