%0 Journal Article %T The Analysis of Rice Total RNA by Capillary Electrophoresis
毛细管电泳技术分析水稻中的总核糖核酸 %A FAN Xing jun %A JIANG Ge %A JIN You xin %A WANG De bao %A
樊兴君 %A 江舸 %A 金由辛 %A 王德宝 %J 色谱 %D 2001 %I %X When the RNase was eliminated through backing at 200 degrees C or treated by diethylpyrocarbonate (DEPC), the total RNA of rice could be separated within 15 min using 1.0%T, 0%C linear polyacrylamide as sieving matrix and 7 mol/L urea as denaturant. The tRNA of rice can be separated into two classes and about nine peaks when high concentrated polyacrylamide sieving matrix (5.0%T, 0%C) was used. This technique could provide one of the rapid and accurate methods for the determination of RNA in plants. %K capillary electrophoresis %K rice total RNA %K rice tRNA
毛细管电泳 %K 水稻 %K 总RNA %K 转移RNA %K 分析 %K 生物化学 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8FA4B8C44E8D375C&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=0B39A22176CE99FB&sid=ED01F5AE50BE09C0&eid=954CE65414DD94CA&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=6