%0 Journal Article
%T Simultaneous Determination of Carotenoids and Chlorophylls in Algae by High Performance Liquid Chromatography<br>高效液相色谱法测定藻类中的类胡萝卜素和叶绿素
%A Yuan Jianping
%A <br>袁建平
%A 张义明
%A 史贤明
%A 龚贤弟
%A 陈峰
%J 色谱
%D 1997
%I 
%X An isocratic reversed-phase HPLC method for the simultaneous determination of carotenoids and chlorophylls in algae is presented. Both carotenoids and chlorophylls were separated by using a C18 column and a mobile phase consisting of dichloromethane/acetonitrile/methanol/water (22.5:9.5:67.5:0.5). The flow rate was 1.0mL/min. These pigments were detected by a UV-VIS absorbance detector set at 450nm. The average recoveries of these pigments by standard addition method were 99.1% for lutein, 98.5% for alpha-carotene, 99.4% for beta-carotene, 100.6% for chlorophyll a and 99.9% for chlorophyll b. The relative standard deviations of these pigments were 2.4% for lutein, 5.6% for alpha-carotene, 6.0% for beta-carotene, 4.1% for chlorophyll a and 4.0% for chlorophyll b. The calibration curves for these pigments gave good linearity over a wide range of 0-50mg/L (r = 0.9992-0.9999). The retention times were 3.0 min for lutein, 4.7 min for chlorophyll b, 6.3 min for chlorophyll a, 14.1 min for alpha-carotene, and 14.7 min for beta-carotene. Using this method, carotenoids and chlorophylls were determined in Spirulina platensis, Chlorella vulgaris and Haematococcus lacustris. Chlorella vulgaris and Haematococcus lacustris samples were ground in a B. Braun homogenizer. Spirulina platensis sample was smashed by supersonic wave. Acetone was used as extractant.
%K high performance liquid chromatography
%K carotenoids
%K chlorophylls
%K algae<br>高效液相色谱法
%K 类胡萝卜素
%K 叶绿素
%K 藻类
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D5A4A3B113E116048&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=0B39A22176CE99FB&sid=76B5E24D6EC46B4B&eid=5E25104E99903E8A&journal_id=1000-8713&journal_name=色谱&referenced_num=16&reference_num=0