%0 Journal Article
%T Preparation of Chitosan-Bonded and Chitosan-Coated-High Performance Liquid Chromatography(HPLC)Packings and Their Applications to Separation of Biopolymers<br>新型壳聚糖液相色谱填料及其在生物分子分离中的应用
%A Wang Junde
%A Wang Yujin
%A Sun Zhaohui
%A <br>王俊德
%A 王宇锦
%A 孙朝晖
%J 色谱
%D 1994
%I 
%X he preparation of chitosan-bonded and chitosan-coated HPLC packings and their applications to the sepa-ration of small peptides,proteins and sugars were reported in the paper.Under isocratic elution,the effectsof mobile phase parameters,such as,pH value and concentration of salts(NH _4)_2SO_4] on the elution of pro-teins were examined.The results showed that the chitosan-bonded pack ing gives a good resolution for thepeptides and both chitosan-silica packings exhibit a certain feature for protein retentions,but they have lessseparation ability for sugars.
%K high performance liquid chromatography
%K chitosan stationary phase
%K small peptide
%K protein
%K monosaccharide<br>壳聚糖
%K 液相色谱填料
%K 生物分子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=BB23D91796E633EC7EFE650F41B5D978&yid=3EBE383EEA0A6494&vid=59906B3B2830C2C5&iid=94C357A881DFC066&sid=170CE8B011EA4FD9&eid=C6EC7357BCACD3A4&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=2