%0 Journal Article
%T Purification of Superoxide Dismutase by High Performance Weak Anion Exchange Chromatography
利用高效弱阴离子交换色谱法纯化超氧化物歧化酶
%A Zheng Ying
%A
郑莹
%A 周信基
%A 李华儒
%J 色谱
%D 1995
%I
%X e procedure of purifying superoxide dismutase was simplified using high performance weak anion ex-change chromatography.In only one chromatographic step following the acetone precipitation,Cu,Zn-super-oxide dismutase from bovine erythrocytes was purified to electrophoretic homogeneity wit h a recovery of86.4%. The specific activity of the enzyme preparation was 7711U/mg,and its purity was increased upto to52-fold.The conditions of chromatographic separation were also discussed in detail.
%K high performance anion exchange chromatography
%K superoxide dismutase
%K bovine blood
离子交换色谱
%K 超氧化物歧化酶
%K 牛血
%K 色谱分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=2BD31EED9264A1594494F927D1CF6EA9&yid=BBCD5003575B2B5F&vid=FC0714F8D2EB605D&iid=E158A972A605785F&sid=6A73B36E85DB0CE9&eid=B78CD622C1934236&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=6