%0 Journal Article
%T Analysis of DNA Restriction Fragments by High Performance Liquid Chromatography(HPLC) on a Nonporous Ion Exchange Column<br>高效液相色谱非多孔型阴离子交换柱分析DNA片段
%A Liao Jie
%A Zhou Jianzhong
%A Qian Xiaohong
%A Dong Fangting
%A Liu Zhihong
%A <br>廖杰
%A 周建忠
%A 钱小红
%A 董芳霆
%A 刘志红
%J 色谱
%D 1996
%I 
%X 建立了高效液相色谱非多孔型阴离子交换柱分离ＤＮＡ片段的方法，用ＴＳＫｇｅｌＤＥＡＥ－ＮＰＲ柱、Ｔｒｉｓ－ＨＣｌ缓冲液（ｐＨ９．０）、１．０ｍｏｌ／ＬＮａＣｌ多阶式线性梯度洗脱，分析了核酸分子量参照物ｐＢＲ３２２ＤＮＡ－ＨａｅⅢ，ＬａｍｂｄａＤＮＡ－ＨｉｎｄⅢ及乙肝病毒基因ＰＣＲ产物，探讨了梯度、流速对分离的影响，方法简便、快速、分辨率高
%K high
%K performance
%K liquid
%K chromatography
%K non
%K porous
%K ion
%K exchange
%K column
%K DNA
%K restriction
%K fragments<br>高效液相色谱法，非多孔型离子交换柱，DNA片段
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=798AA8339DF84A0E10865181B9CF4658&yid=8A15F8B0AA0E5323&vid=F3583C8E78166B9E&iid=B31275AF3241DB2D&sid=8C5DE51F0A009A0F&eid=788931E6318420A3&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=0