%0 Journal Article
%T Cloning and Expression Analysis of Cytosolic Malate Dehydrogenase Gene from Sugarcane<br>甘蔗细胞质型苹果酸脱氢酶基因的克隆及其表达分析
%A XU Jing-sheng
%A QUE You-xiong
%A YAN Ke-wei
%A TANG Wei-qi
%A XU Li-ping
%A GAO San-ji
%A GUO Jin-long
%A DONG Meng
%A CHEN Ru-kai
%A <br>徐景升
%A 阙友雄
%A 颜克伟
%A 唐唯其
%A 许莉萍
%A 高三基
%A 郭晋隆
%A 董萌
%A 陈如凯
%J 热带亚热带植物学报
%D 2012
%I 
%X A full-length cDNA sequence of sugarcane (Saccharum officinarum L.) cytosolic malate dehydrogenase (cMDH) gene, named as Sc-cMDH, was obtained from sugarcane full-length cDNA library by EST sequencing and bioinformatics analysis. The full-length of Sc-cMDH was 1314 bp with 999 bp open reading frame, encoding 332 amino acids. Sc-cMDH shared 86.5%~97.0% of amino acids with cMDH from other 9 species. Sc-cMDH contains typical NAD+ binding motif (T11GAAGQI17) and catalytic motif (I184WGNH188). Real-time PCR analysis showed that the expression of Sc-cMDH was higher in leaves and roots than that in stems of sugarcane.
%K Saccharum officinarum L
%K Cytosolic malate dehydrogenase gene
%K Real-time PCR<br>甘蔗
%K 细胞质型苹果酸脱氢酶基因
%K 实时定量PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=8BF098F6A1C54FF3E022E02449AE674B&yid=99E9153A83D4CB11&vid=A04140E723CB732E&iid=94C357A881DFC066&sid=366B1248A15658C5&eid=FCACFF68346F8D4F&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=19