%0 Journal Article
%T Foreign Protein Expression in Chloroplast Transformation of Phaeodactylum tricornutum<br>叶绿体转化三角褐指藻表达外源蛋白
%A ZHU Cong-cong
%A ZHANG Nai-sheng
%A XIE Wei-hong
%A NIU Ying-fang
%A YANG Wei-dong
%A LIU Jie-sheng
%A LI Hong-ye
%A <br>朱聪聪
%A 张乃升
%A 谢伟红
%A 牛莹芳
%A 杨维东
%A 刘洁生
%A 李宏业
%J 热带亚热带植物学报
%D 2011
%I 
%X To achieve foreign protein expression in the chloroplast of Phaeodactylum tricornutum, a chloroplast transformation vector was constructed. The sequences rns-trnI and trnA-rnl from P. tricornutum chloroplast genome were cloned and used as homologous recombination elements. Chloramphenicol acetyltransferase gene (CAT) expression cassette conferring chloramphenicol resistance was employed as selection marker, and green fluorescent protein gene (GFP) as reporter gene. Based on the TA cloning vector pMD19-T, CAT and GFP expression cassette were cloned in between the two homologous recombination elements. The resultant chloroplast transformation vector was transferred into the chloroplast of P. tricornutum by electroporation method. Transplastomic P. tricornutum cells were obtained upon chloramphenicol selection and reporter GFP could be detected in the chloroplasts, indicating that chloroplast expression system was successfully achieved.
%K 三角褐指藻
%K 叶绿体转化
%K 同源重组
%K 电穿孔
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=5E405F6854E7C56F329646FA37FCEA49&yid=9377ED8094509821&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=866F8A6B640835A7&eid=4E6F5C60B72D9B1C&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=20