%0 Journal Article
%T Sequence and Expression Analysis of Cytoplasmic Copper/zinc Superoxide Dismutase Gene in Rice<br>水稻细胞质铜锌超氧化物歧化酶基因的序列和表达分析
%A WANG Feng
%A WANG Hong-bin
%A WANG Jin-fa
%A <br>王峰
%A 王宏斌
%A 王金发
%J 热带亚热带植物学报
%D 2007
%I 
%X The 698 bp cytoplasmic copper/zinc-superoxide dismutase gene (OsCu/Zn-SOD) was cloned by RT-PCR from young panicles of rice Cpslo17, a wide-compatible rice cultivar (Oryza sativa). DNA sequence analysis indicated that the gene consisted of a full-open reading frame and encoded a protein of 152 amino acid residues. The nucleatide sequence of OsCu/Zn-SOD shares 88% identity to maize Cu/Zn-SOD. No signal peptide was predicted by TargetP and ChloroP. Further analysis of encoding segment of OsCu/Zn-SOD revealed that it contained 8 exons and 7 introns. The putative three-dimensional structure of OsCu/Zn-SOD was predicted by the Swiss-Model server and Cu2 and Zn2 binding sites of OsCu/Zn-SOD were analyzed. RT-PCR results showed differential expression of OsCu/Zn-SOD in leaves of both japnica and indica rice. OsCu/Zn-SOD was expressed highly in panicles during young ear and the flowering stages or in immature seeds, however less in leaves and faintly in callus.
%K RT-PCR<br>水稻
%K 铜锌超氧化物歧化酶
%K 序列分析
%K 基因组结构
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=9CD552077BCFF181&yid=A732AF04DDA03BB3&vid=23CCDDCD68FFCC2F&iid=0B39A22176CE99FB&sid=74011071555EB4E5&eid=F24949CFDB502409&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=13