%0 Journal Article
%T Screening Interaction Proteins of Rubber Elongation Factor (REF) by Yeast Two-hybrid System<br>应用酵母双杂交系统筛选橡胶延长因子REF的互作蛋白
%A ZENG Ri-zhong
%A LI Xian-kun
%A NIE Zhi-yi
%A DAI Long-jun
%A LI Yu
%A DUAN Cui-fang
%A <br>曾日中
%A 李先昆
%A 聂智毅
%A 代龙军
%A 黎瑜
%A 段翠芳
%J 热带亚热带植物学报
%D 2011
%I 
%X The interaction proteins of rubber elongation factor (REF) were screened using yeast two-hybrid system. A bait vector pBD-GAL4-REF without auto-activation was constructed by subcloning ORF cDNA of REF gene into the yeast two-hybrid vector pBD-GAL4, and the pAD-GAL4-2.1 cDNA prey library was prepared with mRNA extracted from the latex of rubber tree (Hevea brasiliensis). The bait and prey vectors were then co-transformed into YRG-2 yeast competent cells by two-step method. Five proteins interacted with REF were identified by yeast two-hybrid screening method, including two of REF family proteins, and small rubber particle protein (SRPP), translation control of tumor protein (TCTP), elicitor-responsive protein and ubiquitin-conjugating enzyme E2. The data suggested that REF could not only interact with high homologous proteins, but also with other proteins, such as TCTP, and E2, etc. These protein-protein interactions might help to revealing the biological function of H. brasiliensis rubber particles.
%K Hevea brasiliensis
%K REF
%K Rubber biosynthesis
%K Yeast two-hybrid system<br>橡胶树
%K 橡胶延长因子REF
%K 橡胶生物合成
%K 酵母双杂交系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=51CDEEA2FDA11B94B16CAD30ED61ADDD&yid=9377ED8094509821&vid=2A8D03AD8076A2E3&iid=94C357A881DFC066&sid=8ACD9060100C26F1&eid=DA74B62FE4348759&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=30