%0 Journal Article %T Cloning and Sequence Analysis of a Novel Cis-prenyltransferases Gene from Hevea brasiliensis
巴西橡胶树顺式异戊烯基转移酶基因cDNA克隆及其序列特征分析 %A LUO Ming-wu %A DENG Liu-hong %A YI Xiao-ping %A ZENG Hui-cai %A XIAO Su-sheng %A
罗明武 %A 邓柳红 %A 易小平 %A 曾会才 %A 肖苏生 %J 热带亚热带植物学报 %D 2009 %I %X Cis-prenyltransferases is the key enzyme to rubber biosynthesis.A cDNA fragment which was highly homologous to the gene encoding cis-prenyltransferases was isolated by screening of a latex subtracted cDNA library.The latex subtracted cDNA library was constructed by suppressive subtractive hybridization(SSH),in which the polyA+RNA of latex from Hevea brasiliensis served as tester,and polyA+RNA of leaves as driver.According to its sequences information,a novel full-length cDNA of 1 156 bp termed R363(GenBank ... %K 巴西橡胶树 %K 抑制消减杂交法 %K 胶乳 %K cis-异戊烯基转移酶 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=29F4CE2CEBE7C44BBAB8687B07B73237&yid=DE12191FBD62783C&vid=BCA2697F357F2001&iid=38B194292C032A66&sid=E089FDF3CDAE8561&eid=CA5852BD1A173B3A&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=12