%0 Journal Article
%T ELECTRQPHORETIC DRIVE: HIGH EFFICIENCY GENE TRANSFER USING A NOVEL APPROACH FOR THE DELIVERY OF FOREIGN GENES INTO RICE CELLS<br>低压脉冲电泳介导的外源基因转化部分酶解的水稻小细胞团
%A Yang Jinshui
%A Ge Koulin
%A Wang Yunzhu
%A Wang Bei
%A Tan Jiazhen
%A <br>杨金水
%A 葛扣麟
%A 王蕴珠
%A 王蓓
%A 谈家桢
%J 热带亚热带植物学报
%D 1992
%I 
%X A new method has been developed to introduced foreign DNA into rice cells. Gene delivery occurs when a low-voltage electrophoresis at long pulse applied to a mixture containing partially digested SCG (small cell groups) and plasmid DNA. Gene-transfer efficiency is evaluated by a detection of GUS activity resulting from the chimeric plasmid DNA. The optimal combination of voltage and pulse produces a frequency of up to 8. 2% for transformation of SCG. The results demonstrates that electrophoretic drive is applicable to introduce foreign genes into plant cells.
%K Rice
%K Small cell groups (SCG)
%K Electrophoresis
%K Gene transfer
%K Stable expression<br>水稻
%K 小细胞团
%K 电泳
%K 基因转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=6C7168AC8BC3BA2CD62A5BD771C107D5&yid=F53A2717BDB04D52&iid=CA4FD0336C81A37A&sid=EDA22B444205D04A&eid=B62E0EEFE746E568&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=0