%0 Journal Article
%T Extraction of Total Protein from Litchi Pericarps and Establishment of Two-dimensional Electrophoresis<br>荔枝果皮总蛋白质提取及双向电泳体系的建立
%A LI Kai-tuo
%A GUO Zhi-xiong
%A PAN Dong-ming
%A ZHONG Feng-lin
%A PAN Teng-fei
%A <br>李开拓
%A 郭志雄
%A 潘东明
%A 钟凤林
%A 潘腾飞
%J 热带亚热带植物学报
%D 2011
%I 
%X The total protein was extracted from Lichi chinensis pericarp by using three methods, such as trichloroacetic acid (TCA)-acetone, acetone and phenol. The results showed that the total protein yield was the highest by phenol extraction method, and protein bands were the most and clear in one dimentional SDS-PAGE. About 870 clear protein spots were detected by two-dimensional electrophoresis (2-DE) and silver staining with isoelectric points at pH 4~8, and relative molecular weight of protein ranged from 15.0 to 85.0 kD.
%K Litchi chinensis Sonn
%K Pericarp
%K Proteome
%K Extraction
%K 2-DE<br>荔枝
%K 果皮
%K 蛋白质组
%K 提取
%K 双向电泳
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=36FC7ECDB815FD053DB20E3D3D2A69CC&yid=9377ED8094509821&vid=2A8D03AD8076A2E3&iid=CA4FD0336C81A37A&sid=CB423C9A71560A74&eid=67969BA850333433&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=12