%0 Journal Article
%T Cloning and Evolutionary Analysis of Homologous Sequences of LEAFY Gene in Capsella bursa-pastoris (Cruciferae)<br>荠菜LEAFY同源基因的克隆与进化分析
%A ZHOU Xian-long
%A WANG Xiao-lan
%A WANG Wu-yuan
%A GE Xue-jun
%A <br>周贤龙
%A 王小兰
%A 王武源
%A 葛学军
%J 热带亚热带植物学报
%D 2006
%I 
%X LEAFY is an important regulator of floral meristems genes in higher plants.To investigate its origin and evolution,a LEAFY homologs with length of 2 866 bp was cloned from Capsella bursa-pastoris genome using the conserved primers from Arabidopsis thaliana LEAFY gene.Sequence analysis indicated three exons with the length of 457 bp,407 bp and 411 bp.These exons encoded a protein of 424 amino acids.The single exon was more than 90% homologous to A.thaliana LEAFY gene,and the amino acid homology was 86%.When compared to Arabidopsis lyrata LEAFY gene,there was 93 nucleotide substitution in the exons of C.bursa-pastoris,and 74.7% of these mutations located at the third position of codon,the amino acid homology reached 90%.Among the three exons,Ds/Dn ratio was 1.9,2.3 and 6.0.Homologous comparison indicated that the C-terminal amino acids were highly conserved among different plant species while the great variation existed in N-terminal.In addition,the Arg mutation located at 346 in CapLFY gene was speculated to be one of main reasons that caused the ecotypes with different flowering time in C.bursa-pastoris.
%K Capsella bursa-pastoris
%K LEAFY gene
%K Cloning
%K Evolutionary analysis<br>荠菜
%K LEAFY基因
%K 克隆
%K 进化分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=03ED093684594632&yid=37904DC365DD7266&vid=F3583C8E78166B9E&iid=0B39A22176CE99FB&sid=A63576421B012172&eid=EFD65B51496FB200&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=2&reference_num=19