%0 Journal Article
%T Relationship between Soil Organic Carbon and Bulk Density in the Rocky Desertification Process of Karst Ecosystem in Guizhou<br>贵州喀斯特石漠化过程中的土壤有机碳与容重关系
%A YAN Jun-hu
%A ZHOU Chuan-yan
%A WEN An-bang
%A LIU Xing-zhao
%A CHU Guo-wei
%A LI Kun
%A <br>闫俊华
%A 周传艳
%A 文安邦
%A 刘兴诏
%A 褚国伟
%A 李坤
%J 热带亚热带植物学报
%D 2011
%I 
%X To achieve foreign protein expression in the chloroplast of Phaeodactylum tricornutum, a chloroplast transformation vector was constructed. The sequences rns-trnI and trnA-rnl from P. tricornutum chloroplast genome were cloned and used as homologous recombination elements. Chloramphenicol acetyltransferase gene (CAT) expression cassette conferring chloramphenicol resistance was employed as selection marker, and green fluorescent protein gene (GFP) as reporter gene. Based on the TA cloning vector pMD19-T, CAT and GFP expression cassette were cloned in between the two homologous recombination elements. The resultant chloroplast transformation vector was transferred into the chloroplast of P. tricornutum by electroporation method. Transplastomic P. tricornutum cells were obtained upon chloramphenicol selection and reporter GFP could be detected in the chloroplasts, indicating that chloroplast expression system was successfully achieved.
%K 喀斯特
%K 石漠化
%K 土壤有机碳
%K 土壤容重
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=5E405F6854E7C56F1DF77C0368139C9C&yid=9377ED8094509821&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=9F8C5EF901EA1E7E&eid=B4E8EA49DAAEB84F&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=1&reference_num=25