%0 Journal Article %T Identification of a Sex-associated RAPD Marker in Cycas tanqingii D.Y.Wang
谭清苏铁性别相关的RAPD标记研究 %A Jing Jian-zhou %A Li Dong-liang %A Jin Hong %A Zhang Yong %A Cao Li-ming %A
景建洲 %A 李东亮 %A 金红 %A 张勇 %A 曹黎明 %J 热带亚热带植物学报 %D 2007 %I %X The total genomic DNA was extracted from half-a-year-old leaflets of the male and female cycads (Cycas tanqingii D. Y. Wang) using an optimized CTAB protocol. The RAPD-PCR amplification conditions were optimized by monofactor and multifactor orthogonal experiments. A total of 160 primers were used for RAPD-PCR amplification and more than 1 450 fragments were obtained from both male and female plants. A fragment of about 500 bp only linked to the female was generated by the S0465 primer, which can be used in sex determination of C. tanqingii. %K RAPD
谭清苏铁 %K 性别 %K 分子标记 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=EEAA36E7201622F1&yid=A732AF04DDA03BB3&vid=23CCDDCD68FFCC2F&iid=38B194292C032A66&sid=FEF02B4635FE8227&eid=974CBB04624305A1&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=1&reference_num=12