%0 Journal Article
%T The Cloning of Replicase Gene of Banana Bunchy Top Virus and the Gene Expression<br>香蕉束顶病毒复制酶基因克隆及转基因表达
%A LIN De-qiu
%A QU Liang-hu
%A ZHANG Hong-ta
%A <br>林德球
%A 屈良鹄
%A 张宏达
%J 热带亚热带植物学报
%D 2004
%I 
%X The DNA was isolated from banana grown in Guangzhou, which was infected with bunchy top virus. 1.1 kb DNA of replicase of banana bunchy top virus (BBTV) was obtained by PCR using this virus DNA astemplates for amplification. The derived DNA of the BBTV replicase was encoded by the C-Terminal of BBTV. It was homologous to 90% with the Australian BBTV. The reformed BBTV replicase was cloned to pBI 121 in the position between CaMV 35S promoter and NOS termination sequence, and a plant expressed carrier was established. Four transgenic platelets with BBTV replicase gene in T0 generation were detected by PCR and Western blot analysis. The ability of these transgenic banana against bunchy top virus is being investigated.
%K Banana bunchy top virus
%K Replicase gene cloning
%K Transgenic banana
%K Disease resistance<br>香蕉束项病毒
%K 复制酶基因
%K 转基因香蕉
%K 抗病性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=B042C0500516D1CE&yid=D0E58B75BFD8E51C&vid=59906B3B2830C2C5&iid=0B39A22176CE99FB&sid=E22B6B8FE86DD8F9&eid=A020552C37306588&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=3&reference_num=16