%0 Journal Article
%T INACTIVATION OF MESOPHYLL PROTOPLASTS USING ACRIDIN ORANGE FOR ASYMMETRIC PROTOPLAST FUSION<br>吖啶橙失活处理应用于不对称融合初步探讨
%A He Yawen
%A Li Gengguang
%A Zhang Lanying
%A <br>何亚文
%A 李耿光
%A 张兰英
%J 热带亚热带植物学报
%D 1996
%I 
%X Inactivation of mesophyll protoplasts from potato cultivars and its wild species was achieved by treatment with acridin orange(AO), under dark culture for 2 days and then exposure to light for 4h. The extent of this inactivation depent on the duration of AO treatment, the AO concentrations and the occasion for light treatment. The inactivation concentrations of tetroploid were higher than those of diploid. Immediate exposure to light after AO treatment showed strongest inactivation effect. Culture exposed to light after 8 days showed almost the same division pattern as control AO-treated in the dark. Small calli formed after fusion of protoplasts in rhodamine (P6G) inactivated potato (Solanum tuberosum) and AO-treated Solanum bulbocastanum.
%K Potato
%K Acridin orange
%K Inactivation treatment
%K Protoplast fusion<br>马铃薯
%K 吖啶橙
%K 失活处理
%K 原生质体融合
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=5AA97395A1A7990A4FEEA05E7BABC871&yid=8A15F8B0AA0E5323&vid=E158A972A605785F&iid=0B39A22176CE99FB&sid=8E6AB9C3EBAAE921&eid=11B4E5CC8CDD3201&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=1&reference_num=2