%0 Journal Article
%T Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator
%A Fernando Rodrigues Ribeiro Teles
%A Duarte Miguel Fran£¿a dos Prazeres
%A Jos¨¦ Luiz De Lima-Filho
%J Sensors
%D 2007
%I MDPI AG
%R 10.3390/s7112510
%X A simple method for electrochemical detection of a synthetic 20-bpoligonucleotide sequence related with dengue virus genome was developed. Acomplimentary DNA probe sequence was electrostatically immobilized onto a glassycarbon electrode modified with chitosan. Electrochemical detection of hybridizationbetween probe and target was performed by cyclic voltammetry, using ferrocene (Fc  ) as ahybridization label. After hybridization, the peak current response of Fc  oxidationincreased around 26%. A higher voltammetric decay rate constant (kd) and a lower half-lifeperiod (t1/2) for the interaction of Fc  with dsDNA compared to those with ssDNAquantitatively characterize the different strengths of interaction with both types of DNA.By combining the simplicity of DNA immobilization onto a chitosan film and suitablevoltammetric detection of hybridization concomitant with ferrocene attachment, a gooddiscrimination between ssDNA and dsDNA was obtained.
%K modified glassy carbon electrode
%K DNA electrochemical biosensor
%K DNA immobilization
%K hybridization indicator
%K chitosan
%U http://www.mdpi.com/1424-8220/7/11/2510