%0 Journal Article
%T PURIFICATION AND CHARACTERIZATION OF CYSTEINE ENDOPEPTIDASE IN COTYLEDONS OF GERMINATED PEANUT (ARACHIS HYPOGAEA L.) SEEDS<br>萌发花生种子子叶肽链内切酶的纯化和性质
%A Bin Jinhu
%A Shen Yun
%A Fu Jiarui
%A <br>宾金华
%A 沈芸
%A 傅家瑞
%J 热带亚热带植物学报
%D 1996
%I 
%X 萌发花生种子子叶的肽链内切酶经硫酸铵沉淀，ＳｅｐｈａｄｅｘＧ－１００凝胶层析，ＤＥＡＥ－纤维素２３阴离子交换层析和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ５０层析，得到纯化的酶，该酶有两条同工酶，分子量分别为５８和５５ＫＤ，Ｋｍ为９．９μｍｏｌ／Ｌ，是半胱氨型肽链内切酶（ＥＣ３．４．２２），对未萌发花生种子的贮藏蛋白没有明显降解作用．
%K Peanut
%K Germinated seed
%K Cysteine endopeptidase
%K Isoenzyme<br>花生
%K 种子萌发
%K 肽链内切酶
%K 同工酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=5AA97395A1A7990AB9397406114BACF2&yid=8A15F8B0AA0E5323&vid=E158A972A605785F&iid=E158A972A605785F&sid=5C3443B19473A746&eid=B9704B40A4225A24&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=1&reference_num=4