%0 Journal Article
%T Chalcone Synthase Gene Cloning in Gerbera hybrida and Expression in E.coli<br>非洲菊查尔酮合酶基因的克隆、序列分析及在大肠杆菌中的表达
%A XIE Xiu-zhi
%A CHEN Zhao-ping
%A WANG Xiao-jing
%A <br>谢修志
%A 陈兆平
%A 王小菁
%J 热带亚热带植物学报
%D 2004
%I 
%X Chalcone synthase (CHS) gene was cloned from the petals of Gerbera hybrida using RT-PCR method in our experiment. The sequence of the cDNA coding region was 1 197 bps, encoding a protein of 398 amino acids sharing 99% identical to CHS1 cDNA reported by Helariutta. The gene was fused in expression vector pET32a and was efficiently expressed by transforming into E. coli BL21(DE3) with IPTG induction.
%K Gerbera hybrida
%K Chalcone synthase
%K Sequence analysis
%K Gene expression<br>非洲菊
%K 查尔酮合酶
%K 序列分析
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=D459C2F6EDF5CE4C&yid=D0E58B75BFD8E51C&vid=59906B3B2830C2C5&iid=94C357A881DFC066&sid=6D6BFCF0101BC091&eid=E57FE519484CFB70&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=9&reference_num=15