%0 Journal Article
%T A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array
%A Dongjin Lee
%A Janet Ondrake
%A Tianhong Cui
%J Sensors
%D 2011
%I MDPI AG
%R 10.3390/s111009300
%X We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I¨CV measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4¨C12 nA/mM for channel lengths of 5¨C20 ¦Ìm, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.
%K biosensor array
%K nanoparticle
%K conductometric sensor
%K microsensor array
%K glucose sensor
%U http://www.mdpi.com/1424-8220/11/10/9300