%0 Journal Article %T Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats %A Kim E. Sapsford %A Samantha Spindel %A Travis Jennings %A Guoliang Tao %A Robert C. Triulzi %A W. Russ Algar %A Igor L. Medintz %J Sensors %D 2011 %I MDPI AG %R 10.3390/s110807879 %X The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took %K quantum dot (QD) %K nanocrystal (NC) %K semiconductor %K bioconjugation %K sensor %K multiplex %K immunoassay %K sulfhydryl chemistry %U http://www.mdpi.com/1424-8220/11/8/7879