%0 Journal Article
%T Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles
%A Harri H£¿rm£¿
%A Juha Toivonen
%A Juhani T. Soini
%A Pekka H£¿nninen
%A Wolfgang J. Parak
%J Sensors
%D 2011
%I MDPI AG
%R 10.3390/s111211335
%X Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.
%K colloidal nanoparticles
%K quantum dots
%K time-gated fluorescence
%K sensing
%K bioaffinity assays
%U http://www.mdpi.com/1424-8220/11/12/11335