%0 Journal Article
%T Simplistic Attachment and Multispectral Imaging with Semiconductor Nanocrystals
%A Travis L. Jennings
%A Robert C. Triulzi
%A Guoliang Tao
%A Zachary E. St. Louis
%A Sara G. Becker-Catania
%J Sensors
%D 2011
%I MDPI AG
%R 10.3390/s111110557
%X Advances in spectral deconvolution technologies are rapidly enabling researchers to replace or enhance traditional epifluorescence microscopes with instruments capable of detecting numerous markers simultaneously in a multiplexed fashion. While significantly expediting sample throughput and elucidating sample information, this technology is limited by the spectral width of common fluorescence reporters. Semiconductor nanocrystals (NC¡¯s) are very bright, narrow band fluorescence emitters with great potential for multiplexed fluorescence detection, however the availability of NC¡¯s with facile attachment chemistries to targeting molecules has been a severe limitation to the advancement of NC technology in applications such as immunocytochemistry and immunohistochemistry. Here we report the development of simple, yet novel attachment chemistries for antibodies onto NC¡¯s and demonstrate how spectral deconvolution technology enables the multiplexed detection of 5 distinct NC-antibody conjugates with fluorescence emission wavelengths separated by as little as 20 nm.
%K quantum dot
%K nanocrystal
%K IHC
%K immunohistochemistry
%K spectral imaging
%K multiplexing
%K conjugation
%K antibody
%U http://www.mdpi.com/1424-8220/11/11/10557