%0 Journal Article
%T Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells
%A CHEN Zhiling
%A YU Yi
%A LIU Lin
%A XIA Guixian
%A <br>CHEN Zhiling
%A YU Yi
%A LIU Lin
%A XIA Guixian
%J 自然科学进展
%D 2007
%I 
%X The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48. The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245?374 and aa 518?646). Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through G1 to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells.
%K tobacco BY-2 suspension cells
%K NtCDC48
%K spindle
%K cell cycle<br>tobacco
%K BY-2
%K suspension
%K cells
%K NtCDC48
%K spindle
%K cell
%K cycle.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=504AF8C1E5476CA7C4EC9DF6FEAC14AC&aid=802EC38DE6269214D7FC26B71C940A04&yid=A732AF04DDA03BB3&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=3F0AF5EDBC960DB0&eid=F1177A9DF1349B63&journal_id=1002-008X&journal_name=自然科学进展&referenced_num=0&reference_num=0