%0 Journal Article
%T Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation
%A Joshua A. Hagen
%A Sang N. Kim
%A Burhan Bayraktaroglu
%A Kevin Leedy
%A Jorge L. Ch¨¢vez
%A Nancy Kelley-Loughnane
%A Rajesh R. Naik
%A Morley O. Stone
%J Sensors
%D 2011
%I MDPI AG
%R 10.3390/s110706645
%X Zinc oxide field effect transistors (ZnO-FET), covalently functionalized with single stranded DNA aptamers, provide a highly selective platform for label-free small molecule sensing. The nanostructured surface morphology of ZnO provides high sensitivity and room temperature deposition allows for a wide array of substrate types. Herein we demonstrate the selective detection of riboflavin down to the pM level in aqueous solution using the negative electrical current response of the ZnO-FET by covalently attaching a riboflavin binding aptamer to the surface. The response of the biofunctionalized ZnO-FET was tuned by attaching a redox tag (ferrocene) to the 3¡¯ terminus of the aptamer, resulting in positive current modulation upon exposure to riboflavin down to pM levels.
%K aptamer
%K biomolecular detection
%K ZnO-FET
%K sensor
%K riboflavin
%K selectivity
%K label-free
%K biofunctionalization
%U http://www.mdpi.com/1424-8220/11/7/6645