%0 Journal Article
%T Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene
%A Rosie E. Bradshaw
%A Zhilun Feng
%A Arne Schwelm
%A Yongzhi Yang
%A Shuguang Zhang
%J Toxins
%D 2009
%I MDPI AG
%R 10.3390/toxins1020173
%X Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, DotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing DotC, or with a DotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of DotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.
%K aflatoxin biosynthesis
%K Dothistroma septosporum
%K major facilitator superfamily
%K toxin transporter
%K red-band needle blight
%U http://www.mdpi.com/2072-6651/1/2/173