%0 Journal Article
%T Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods
%A Mohamad Kamal Abdul Kadir
%A Ibtisam E. Tothill
%J Toxins
%D 2010
%I MDPI AG
%R 10.3390/toxins2040382
%X An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 ¦Ìg¡¤L-1for fumonisins. This was then transferred to the surface of a bare gold screen-printed electrode (SPGE) and detection was performed by chronoamperometry, monitoring the reaction of 3,3¡¯,5,5¡¯-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2) catalysed by HRP at £¿100 mV potential vs. onboard Ag-AgCl pseudo-reference electrode. The immunosensor exhibited detection limit of 5 ¦Ìg¡¤L£¿1 fumonisins with a dynamic range from 1 ¦Ìg¡¤L£¿1¨C1000 ¦Ìg¡¤L£¿1. The sensor also performed well in extracted corn samples.
%K fumonisins
%K mycotoxins
%K screen-printed gold electrode
%K electrochemical immunosensor
%U http://www.mdpi.com/2072-6651/2/4/382