%0 Journal Article
%T Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
%A Eleonora Fusi
%A Raffaella Rebucci
%A Chiara Pecorini
%A Anna Campagnoli
%A Luciano Pinotti
%A Francesca Saccone
%A Federica Cheli
%A Stig Purup
%A Kristen Sejrsen
%A Antonella Baldi
%J Toxins
%D 2010
%I MDPI AG
%R 10.3390/toxins2061265
%X The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of ¦Á-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM ¦Á-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that ¦Á-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.
%K ochratoxin A
%K ¦Á-tocopherol
%K DNA damage
%K fibroblasts
%K swine
%U http://www.mdpi.com/2072-6651/2/6/1265