%0 Journal Article
%T An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System
%A Gustavo A. Alonso
%A Rocio B. Dominguez
%A Jean-Louis Marty
%A Roberto Mu£¿oz
%J Sensors
%D 2011
%I MDPI AG
%R 10.3390/s110403791
%X An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Multi-commuted Flow Analysis system (MCFA) was selected and developed to carry out assays with an improved inhibition method to detect the insecticides chlorpyrifos oxon (CPO), chlorfenvinfos (CFV) and azinphos methyl-oxon (AZMO). The system manifold comprised a peristaltic pump, a set of seven electronic valves controlled by a personal computer electronic interface and software based on LabView£¿ to control the sample dilutions into the cell. The inhibition method consists in the injection of the insecticide when the enzyme activity has reached the plateau of the current; with this method the incubation time is avoided. A potentiostat was developed to measure the response from the enzymatic biosensor. Low limits of detection of 10 nM for CPO, CFV, and AZMO were achieved.
%K biosensor
%K enzyme inhibition
%K flow system
%K multi-commuted
%K organo-phosphorus insecticides
%K potentiostat
%U http://www.mdpi.com/1424-8220/11/4/3791