%0 Journal Article
%T Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator
%A John T. Gohring
%A Xudong Fan
%J Sensors
%D 2010
%I MDPI AG
%R 10.3390/s100605798
%X We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.
%K HIV
%K CD4
%K CD8
%K ring resonator
%K optical biosensor
%K label free
%U http://www.mdpi.com/1424-8220/10/6/5798