%0 Journal Article
%T Amlodipine inhibits matrix metalloproteinases expression and secretion in mouse macrophage
%A Yamin CAO
%A Shiwen WANG
%A Haiyun WU
%A <br>YaminCAO
%A Shiwen WANG
%A Haiyun WU
%J 老年心脏病学杂志(英文版)
%D 2005
%I Science Press
%X Objective To investigate whether the calcium channel blocker umlodipine could inhibit macrophage matrix metalloproteinase-2 (MMP-2) and matrix melalloproteinasc-9 (MMP-9) expression and secretion. Methods Peritoneal macrophages were isolated from BALB/C mice and incubated with low (5|ig/L ), middle ( 1'p.g/L) and high (30?u,g/L) concentrations of amlodipine. or in the medium alone (controls) for 24 hours, and the expression and secretion of MMP-2 and MM-9 of the cells were analyzed by RT-PCR and gelatin zymography. Results Compared with controls, amlodipine at low concentration had no significant effects on the expression and secretion of either MMP-2 and MMP-9 (P>0.05) at middle concentrationit it could inhibited MMP-2 and MMP-9 expressions completely and significantly reduced the secretion of MMP-9 (P<().()5); but it had no effect on the secretion of MMP-2. At high concentration it also inhibited MMP-2 and MMP-9 expression completely. Conclusion Amlodipine at 15 ig/L inhibited the expression of MMP-2 and MMP-9 and reduced the secretion of MMP-9, suggesting that amlodipine may stabilize atherosclerotic plaque.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=AD36F34DDC3BA7B0&jid=4DC702B3A2386A4814E2FF9CFB799B27&aid=B78E3B64082D710376CB4363E5B558CC&yid=2DD7160C83D0ACED&vid=0B39A22176CE99FB&iid=E158A972A605785F&sid=C812B90E96151014&eid=0C191C6ECF79047F&journal_id=1671-5411&journal_name=Journalofgeriatriccardiology:JGC&referenced_num=0&reference_num=8